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Hyaluronic Acid

Hyaluronic acid is an anionic, nonsulfated glycosaminoglycan distributed widely throughout connective, epithelial, and neural tissues.
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Introduction

Hyaluronic acid is an anionic, nonsulfated glycosaminoglycan distributed widely throughout connective, epithelial, and neural tissues. It is unique among glycosaminoglycans in that it is nonsulfated, forms in the plasma membrane instead of the Golgi apparatus, and can be very large, with its molecular weight often reaching the millions. One of the chief components of the extracellular matrix, hyaluronan contributes significantly to cell proliferation and migration, and may also be involved in the progression of some malignant tumors. Hyaluronic acid is also a component of the group A streptococcal extracellular capsule, and is believed to play a role in virulence.

Item Standard Result
Appearance White or almost white,very hygroscopic powder or fibrous aggregate Complied
Solubility Sparingly soluble or soluble in water,practically insoluble in acetone and in anhydrous ethanol Complied
A.Infrared absorption spectrophotometry Complied
Identification Bit gives reaction(a)of sodium Complied
Sodium hyaluronate >95% 99.30%
Appearance of Solution A600nmW Complied
Nucleic Acids A260nmW 0.5 Complied
PH 5.0-8.5 6.4
Molecular weight Measured value 0.16M Da
Intrinsic viscosity Measured value 4.13dL/g
Protein ≥0.1% 0.04%
Loss on drying ≤10.0% 6.70%
Chlorides ≤0.5% Complied
Iron ≤80ppm <80ppm
TAMC <100CFU/g Complied
Staphylococcus aureus Negative/g Complied
Pseudomonas aeruginosa Negative/g Complied
ConclusionComplied with EP9.0

Functions and Applications

1. Hyaluronic Acid could be used in eye surgery, such as corneal transplantation, cataract surgery, glaucoma surgery, and surgery to repair retinal detachment    
2. Hyaluronic Acid can also be used to treat osteoarthritis of the knee. They are administered as a course of injections into the knee joint, which are believed to supplement the viscosity of the joint fluid, thereby lubricating the joint, cushioning the joint, and producing an analgesic effect.
3. Hyaluronic Acid has also been used in the synthesis of biological scaffolds for woundhealing applications. These scaffolds typically have proteins such as fibronectin attached to the Hyaluronic Acid to facilitate cell migration into the wound.    
4. Hyaluronic Acid is also used in antiadhesive products, which are widely used in pelvic and abdominal surgery to prevent postoperative adhesions.

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